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Problem with "run_analysis_gated" #15

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CeliaJuarillo opened this issue Feb 8, 2024 · 0 comments
Open

Problem with "run_analysis_gated" #15

CeliaJuarillo opened this issue Feb 8, 2024 · 0 comments

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@CeliaJuarillo
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Hi,
I'm trying to run "run_analysis_gated" but I keep encountering the same error:
"Markers used for SCAFFoLD: CD45RA, CD8, CCR7, CD3, CD4, CD25, CD56, CD127, HLADR, CD38"
[1] "Using as reference Panel_Pep_Javier T+NK 230124-BL_01 CNT-ALL BL-1 CONTROL_Unmixed.fcs.clustered.txt"
[1] "Downsampling to 1000 events"
[1] "Processing /home/cjuarez/Escritorio/spectral/cyclone_new/outputs/scaffold/Panel_Pep_Javier T+NK 230124-BL_01 CNT-ALL BL-1 CONTROL_Unmixed.fcs.clustered.txt"
[1] "Running with Edge weight: 4.000000"
Error in [.data.frame(tab, , col.names) : undefined columns selected
Calls: ... process_data -> build_graph -> as.matrix -> [ -> [.data.frame
Además: Warning messages:
1: replacing previous import ‘flowCore::normalize’ by ‘igraph::normalize’ when loading ‘scaffold’
2: replacing previous import ‘plyr::rename’ by ‘reshape::rename’ when loading ‘scaffold’
3: replacing previous import ‘plyr::round_any’ by ‘reshape::round_any’ when loading ‘scaffold’
4: '.local' is deprecated.
Use 'keyword' instead.
See help("Deprecated")

I tried using it with less and different markers in case it has something to do with some problem with the values for one of the columns of markers, but then I obtain this error:
Error in apply(tab[, col.names], 1, function(x) { :
dim(X) must have a positive length
Además: Warning message:
'.local' is deprecated.
Use 'keyword' instead.
See help("Deprecated")

I don't know what it's happening my code is this:
scaffold:::run_analysis_gated("/home/cjuarez/Escritorio/spectral/cyclone_new/outputs/scaffold", #scaffold_dir,
"Panel_Pep_Javier T+NK 230124-BL_01 CNT-ALL BL-1 CONTROL_Unmixed.fcs.clustered.txt", #ref_dataset_file
c("CD8", "CCR7", "CD3", "CD4", "CD45RA", "CD25", "CD56", "CD127", "HLADR", "CD38"), #marker_metadata
5)

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