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The filter subcommand
Guanliang MENG edited this page Jun 22, 2023
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You can use this subcommand to filter your raw fastq data, and subsample part of the resulting clean data (via the --data_size_for_mt_assembly
option).
$ mitoz filter -h
usage: mitoz filter [-h] --fq1 <file> [--fq2 <file>] [--phred64] [--outprefix <str>]
[--fastq_read_length <INT>] [--data_size_for_mt_assembly <float1>,<float2>]
[--filter_other_para <str>] [--thread_number <int>] [--workdir <directory>]
[--workdir_done <directory>] [--workdir_log <directory>]
Filter input fastq reads.
optional arguments:
-h, --help show this help message and exit
--fq1 <file> Fastq1 file
--fq2 <file> Fastq2 file
--phred64 Are the fastq phred64 encoded? [False]
--outprefix <str> output prefix [out]
--fastq_read_length <INT>
read length of fastq reads, used to split clean fastq files. [150]
--data_size_for_mt_assembly <float1>,<float2>
Data size (Gbp) used for mitochondrial genome assembly, usually between 3~8 Gbp is
enough. The float1 means the size (Gbp) of raw data to be subsampled, while the float2
means the size of clean data should be >= float2 Gbp, otherwise MitoZ will stop to run.
When only float1 is set, float2 is assumed to be 0. Set float1 to be 0 if you want to
use ALL raw data. [5,0]
--filter_other_para <str>
other parar. []
--thread_number <int>
thread number [4]
--workdir <directory>
working directory [./]
--workdir_done <directory>
done directory [./done]
--workdir_log <directory>
log directory [./log]
About:
Commands:
- The -all- subcommand
- The -filter- subcommand
- The -assemble- subcommand
- The -findmitoscaf- subcommand
- The -annotate- subcommand
- The -visualize- subcommand
Usages:
- Installation
- Tutorial
- Extending MitoZ-s database
- Batch processing of many samples
- Known issues
- FAQ
- Some important intermediate files
- Upload to GenBank
MitoZ-tools:
- Overview: The -mitoz tools- command
- The -mitoz-tools--group_seq_by_gene- command
- The -mitoz tools bold_identification- command
- The -mitoz tools circle_check- command
- The -mitoz tools gbfiletool- command
- The -mitoz tools gbseqextractor- command
- The -mitoz tools msaconverter- command
- The -mitoz tools taxonomy_ranks- command